Comments Off on Safe-Decon releases second White Paper: Adding Water inside Autoclavable Bag results in Inconsistent Decontamination
For over two decades, I have worked at the intersection of biomedical engineering, thermal sterilization science, and autoclave systems development. During this time, I have observed the persistence of a flawed protocol in many biosafety laboratories: the addition of water to sealed biohazard bags under the belief that it enhances autoclave decontamination. This method contradicts well-established thermodynamic principles and fails under scrutiny when tested against modern standards of steam penetration and sterilization consistency. This white paper presents empirical evidence and engineering analysis demonstrating why this method is ineffective and potentially hazardous, and why it must be replaced by systems engineered for true sterilization control.