Studies

Safe-Decon releases second White Paper: Adding Water inside Autoclavable Bag results in Inconsistent Decontamination


For over two decades, I have worked at the intersection of biomedical
engineering, thermal sterilization science, and autoclave systems
development. During this time, I have observed the persistence of a flawed
protocol in many biosafety laboratories: the addition of water to sealed
biohazard bags under the belief that it enhances autoclave
decontamination. This method contradicts well-established thermodynamic
principles and fails under scrutiny when tested against modern standards of
steam penetration and sterilization consistency. This white paper presents
empirical evidence and engineering analysis demonstrating why this
method is ineffective and potentially hazardous, and why it must be
replaced by systems engineered for true sterilization control.

White Paper  https://safedeconinc.com/wp-content/uploads/2025/07/Water-in-Bag-White-Paper.pdf